 Abstract nanotechnology is used to overcome fundamental flaws in today's marketed pharmaceuticals that hinder therapy, such as limited solubility and rapid release of drugs into the bloodstream. In both human and animal researchers, melatonin was shown to regulate glucose levels. Despite the fact that melatonin is quickly transported through the mucosa, its susceptibility to oxidation creates a challenge in achieving the desired dose. Additionally, due to its variable absorption and poor oral bioavailability requires the development of alternative delivery methods. The study aimed to synthesize melatonin-loaded chitosand lecithin, mel-C-L, nanoparticles to be evaluated in the treatment of streptozotosin, STZ, induced diabetes in rats. The antioxidant, anti-inflammatory, and cytotoxicity properties of nanoparticles were estimated to determine the safety of manufactured nanoparticles for in vivo studies. In addition, mel-C-L nanoparticles were administered to rats for eight weeks after inducing hyperglycemia. This article was authored by Habiba Orlo, Mariam Abdulaziz, Mariam Mustafa, and others. We are article.tv, links in the description below.