 This study found that the ethyl acetate fraction of Montingia calibura L. Lees was the most effective in inhibiting the growth of Staphylococcus aureus bacteria. Additionally, it identified one active spot on the bio-autography test, suggesting that this spot may contain compounds responsible for the antimicrobial activity. This article was authored by Risky H. Mowarty, Nanix Cilistiani, Nercocena Nercocena, and others.