 Abstract lacticasobacillus paracasi is an economically important bacterial species used in the food industry and as a probiotic. Here, we investigate the roles of N6 methyladenine, 6mA, modification in L. Paracasi using multiomics and high throughput chromosome confirmation capture, HI-C, analyses. The distribution of 6MAR modified sites varies across the genomes of 28 strains and appears to be enriched near genes involved in carbohydrate metabolism. A PGLX mutant, defective in 6mA modification, shows transcriptomic alterations but only modest changes in growth and genomic spatial organization. This article was authored by Jie Jiao, Meng Zhong, Wen Yanghui, and others.