 In order to grow, they'll need very specific environmental conditions, proper temperature, food, energy and humidity. When growing cells in the lab, we have to create these specific conditions using culture media. Solid culture media is a mixture of nutrient and agar that is poured to the factory plate. In this experiment, we will demonstrate the steps that are used for the preparation of potato dextrose agar for the growth of fungi. This is a potato dextrose agar powder. Always check a lot number and expiry date. The upper panel lists the composition of the potato dextrose agar. The potato dextrose agar is composed of potato infusion, dextrose and agar. This is a way bot. Place the way bot into the analytical balance on the balance port. Press the tear key to zero out the way bot. Use a lab scoop to add 3.9 gram of potato dextrose agar to the way bot. Potato dextrose agar that is 3.9 gram to the beaker. Now add distilled water to the beaker. The next step is stirring. We add magnetic stirrer to the beaker. Stir the solution so that all of the visible clumps will have been broken down. After the stirring, we will adjust the pH of potato dextrose agar media to 5.5 in order to enhance the growth of fungi and to slightly inhibit the growth of bacteria that are commonly found as environmental contaminants. We will adjust the pH of the solution by adding 2-3 drops of 0.1 normal phosphoric acid. We will transfer the media to the elenmer flask. This is elenmer flask. It is commonly known as a conical flask. A autoclave to sterilize the media. The heat comes from the autoclave will help the agar to properly dissolve in the water. Use the button to power on the autoclave. Distilled water to the level of indicator line. Place the media into the bucket. Insert the bucket into the autoclave of the autoclave and turn the handle to create airtight sea. Use the control panel to set the temperature, time and pressure. For the autoclave, the temperature is 121 degrees. The time is 15 minutes and the pressure is 15 psi. Start the autoclave. While it's running, enter the date, time and operation details in the logbook. Once the cycle ends, the pressure gauge reaches to 0 psi. Slowly open the lid. Remove the media from the autoclave.