 This paper presents a new LC-MS-slash-MS method for the simultaneous identification and quantification of cyanotoxins with both hydrophilic and lipophilic properties in edible bivalves. The method includes 17 cyanotoxins comprising 13 microcystins, MCs, nodularin, nod, anatoxin A, ATXA, Homoanatoxin, HATX, and Cylindrospermopsin, CWIN. The method was validated using spiked muscle samples in the quantification range of 3.12 to 200 μg slash kg. The method showed limitations for MCLF, R2 equals 0.94, MCLA, R2 less than or equal to 0.98, and MCLW, R2 less than or equal to 0.98. Recovers for ATXA, HATX, CYN, NOD, MCLF and MCLW were lower than desired, less than 70%. Despite these limitations, the validation results demonstrated the method specificity and robustness for the investigated parameters. This article was authored by Julio Cesar Espana-Emortegui, Heidi Piccar, Mark Dennis Chico-Ritrado, and others. We are article.tv, links in the description below.