 The study evaluates six novel nucleic acid extraction technologies from different developers using blinded panels of stool, sputum, and blood spiked with variable amounts of quality-controlled DNA and or RNA-based microbes to determine their diagnostic accuracy, suitability for resource-limited settings, RLS, and cost estimates. The technologies were ranked based on overall diagnostic accuracy and other metrics such as sample input and output volumes, total processing time, user-required manual steps, and cost estimates. The study aims to identify component technologies for incorporation of reliable and affordable sample preparation with pathogen-nucleic acid amplification, flash detection into an integrated platform suitable for RLS, towards reducing infectious disease-associated morbidity and mortality globally. This article was authored by Shivani G. Biel, Jason Cantera, Maureen H. Diaz, and others.