 Bacillus Sirius Strain GGBSU-1, Produce Mirabilis Strain TL14-1, and Klebsiella varicola strain AUH, KAM-9 were isolated from ferruginous ultisols and characterized by 16S RNA gene sequencing. These bacteria were found to be safe for use as biopriming agents for rice seeds grown in ferruginous ultisols. After three hours of priming, the seeds exhibited increased germination rates and better root development when compared to untreated control seeds. After 12 hours of priming, the seeds also showed increased shoot length and biomass production. Scanning electron microscopy revealed greater bacterial colonization in the primed seeds. Priming with these bacteria improved the growth properties of rice seeds in ferruginous ultisols, suggesting their potential use as biopriming agents for rice cultivation in such soils. This article was authored by Shahid Aymusa.