 The biosynthesis method is thought to be a clean, nantastic, and environmentally friendly way to produce silver nanoparticles. It has been shown to be effective in controlling the size of these particles, with smaller particles being formed when certain conditions are met. These conditions include the use of a specific fungus, fusarium oxysporum, at a particular temperature and pH level, along with a specific amount of silver nitrate and fungal age. This process also produces antimicrobial activity against both the scuricicoli and staphylococcus aureus, with the highest zone of inhibition being 2 mm and 1.6 mm, respectively, at 80 l of silver nanoparticles. Additionally, Cytotoxicity testing shows that the silver nanoparticles have an IC50 value of 121.23 gcm3. This article was authored by Sheriff Musa Hussaini, Tohari Silla, and Hende Antia.