 the study examined the effects of overexpressing four genes involved in the synthesis of isobutonal in Saccharimus' cerevisiae. The results showed that overexpression of these genes resulted in an increase in isobutonal production in S. Cerevisiae. Additionally, the study found that the addition of amino acids to the culture medium improved isobutonal production in the reference strain, but did not improve it in the ILV2, ILV3, ILV5 overexpression strain. This suggests that other factors beyond the activity of the genes are limiting isobutonal production in this strain. This article was authored by Kahumar Kaisa, Keeland Brandt Morton C., Borodina Arena, and others.