 Hello and welcome to this presentation. My name is James Sakoko. The title of my presentation is Molecular Epidemiology of Brucellar Species in Mixed Livestock Human Ecosystems in Kenya and Tanzania. Brucellar species cause infection in animals and humans and results into socioeconomic losses. Several studies have reported the presence of Brucellar antibodies in Kenya and Tanzania, but information on circulating species of Brucellar within the region still remains insufficient. Therefore, we collected a total of 1,051 samples from cattle, sheep, goats, camels, pigs, and humans, from Marsabit and the Narrow Counties in Kenya, as well as Northern Tanzania. We extracted DNA from these samples and tested the DNA for the presence of the genus Brucellar, as well as Brucellar botas and Brucellar militancies using real-time PCR technique. Our results shows that Brucellar botas and Brucellar militancies are circulating in human population, as well as all the livestock species, except pigs that only tested positive for Brucellar botas. The distribution of Brucellar species varied across the different regions, with Northern Tanzania and Narrow having a higher proportion of Brucellar militancies as opposed to Brucellar botas, while Marsabit region had a higher proportion of Brucellar botas. We did not find any significant association between Brucellar botas and sheep and goats, while Brucellar militancies didn't have any significant association with cattle. We found significant association between currents of abortion and the presence of Brucellar botas, while Brucellar militancies were associated with the return placenta in animals. People within the age category between 21 and 40 had an elevated positivity rate. This is mainly due to their increased role in taking care of animals. So in summary, we found zoonotic species of Brucellar circulating the different animal species, suggesting that all these animals would be playing a role in transmitting Brucellar to humans. Therefore, we commend a multidisciplinary approach that target all the different lives of species to help in creating public awareness and contribute to the reduction of the existing risks, such as improper handling and disposal of aborted materials, return placenta, drinking of raw milk, as well as mixing hearts that could perpetuate the transmission of Brucellar within the population. Otherwise, if nothing is done, then we still have continued transmission of Brucellar that results into socioeconomic losses. Thank you very much for your attention.