 Ancosircheosis is a tropical disease caused by the parasitic nematode Ancosirca vulvulus. It is targeted for elimination through the use of ivermectin mass administration. This drug kills the microfilare and temporarily halts their reproduction by the microfilare. To assess the effectiveness of this treatment, we genotyped 436 microfilare from 10 people each in Ituri, Democratic Republic of the Congo, DRC, and Mariti County, South Sudan. These samples were taken both before and four to five months after the treatment. Our analyses revealed 52 and 103 mitochondrial DNA haplotypes among the microfilare from DRC and South Sudan, respectively. However, only a small number of these haplotypes were shared between the two populations. Furthermore, our analyses indicated that the genetic diversity in DRC and South Sudan was underestimated due to the limited number of samples. Additionally, our analyses showed that the per-person adult worm burden was higher in South Sudan than in DRC. Finally, our anal. This article was authored by Shannon M. Hedkey, Young Jun Choi, Anusha Code, and others. We are article.tv, links in the description below.