 The study aims to evaluate genetic resources for future sweet cherry breeding programs and fruit production stability by using DNA molecular genetic analyses, eliminating duplications, and characterizing genetic relationships among 123 current, old, and local sweet cherry cultivars from checked genetic resources. The results show that the accessions can be clustered into three main clusters and 10 subgroups based on genealogical and geobotanical characteristics, providing valuable information for breeding programs and fruit production stability. This article was authored by Joseph Patzak, Elena Henekova, Frantisek Parsdine, and others.