 room temperature crystallography has revealed that small molecules bind differently than they do at cryogenic temperatures. This includes different binding poses, changes in solvent, and the discovery of new binding sites. These findings suggest that crystals grown at cryogenic temperatures may not be representative of the true structure of a protein ligand system, and that room temperature crystallography could provide a more accurate representation of the protein's conformation. This article was authored by Tema-Scased Melman, Justin TBL, Saida Mariam Azim, and others.