Using Droplet Digital™ PCR for Cancer and Liquid Biopsy Studies





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Published on Dec 31, 2013

For more info, visit http://www.bio-rad.com/yt/4/QX200-Dro....
Droplet Digital PCR (ddPCR™) technology is being used to identify cancer subtypes, optimize drug treatment plans, monitor residual disease, and study tumor evolution. ddPCR assays offer advantages in the field of liquid biopsies, enablingto the measurement of circulating nucleic acids (cfDNA) and circulating tumor cells (CTCs) in blood. ddPCR can detect rare tumorigenic mutations in a high background of normal DNA, routinely down to 0.01% and often further. Dr Dawne Shelton discusses the techniques and methods that will enable you to begin your own investigations.

The QX200™ Droplet Digital PCR system, Bio-Rad's second-generation digital PCR system, provides absolute quantification of target DNA or RNA molecules using EvaGreen or TaqMan hydrolysis probes, yielding unmatched sensitivity and precision for a wide variety of applications.

Presenter: Dawne Shelton PhD, Senior Scientist
Dr. Shelton earned her PhD in oncological sciences from the University of Utah, Huntsman Cancer Institute. She has investigated different aspects of the Wnt pathway in colon cancer, studied the origins of the different subtypes of breast cancer, and examined uterine cancer in a clinical cancer research lab. She is now a senior scientist at the Digital Biology Center at Bio-Rad Laboratories, focusing on developing applications and collaborations for ddPCR in the cancer field.


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