 Sleep is essential to our life. Perhaps it's almost important as breathing and eating. Despite the intensive study, the mechanism of sleeping is still not very clear. About 20% of adults may have chronic insomnia that feel difficulty falling asleep and maintain sleep. The insomnia can substantially influence the quality of life, may cause fatigue, low efficiency, and may even cause depression and anxiety. There are several approaches that can treatment for sleep disorders. For example, a gold-sleep habit, some psychology therapy. But these measures may not sometimes not be so effective. Most often, we have to take a drug. The problem with drug is that it can not only affect sleep, but also can affect many brain function, because it can affect most of the neurons throughout the brain. So the question is, can we find an approach that can specifically manipulate the sleep without affecting other brain functions? So the sleep actually may have two major facets. One is the survival sleep. The other is the process of our body seem to slow down. So we have a good rest and refreshing. The other factor is REM sleep. And during sleep, the brain seems not rested with activity just like a wake. And also we can with rapid eye movement. So our sleep will always start with a slow sleep, then fall by REM, and then back to slow sleep again. So this cycle can repeat several times. Traditionally, scientists actually found that the brain has many other systems that can maintain our brain in a wake state. But we don't know whether our brain has a specific brain circuit that can actively control sleep. So that's the question that we want to address. Recently, scientists have developed approaches that can specifically activate the neuron network so that we can specifically control animal behavior. This is by we call the optogenetics that we can express the gene in the specific circuit so that we can control the specific behaviors. In these measures, we found that when we not stimulate the garbage neuron in our LTD, or tiny brain area in the brain, we found that it can induce freezing behavior. It's kind of in that figure. It's just like the master sees a cat response. It cannot move. Then we designed another experiment that we expose TMT that are components of the cat balls that the master is very afraid of. So we can induce freezing response. But if we see the same LTD for another population neuron, the mass are no more afraid of TMT. They can move around just to see. But when we stop the simulation, the mice are freezing again. So use this method, we use proud of the sleeping mechanism just to see here. We found that if the mice in the saliva sleep when you stimulate the acetylcholine neuron, you can awake the animal. But we can never induce the REM sleep. People believe that this is related to REM sleep. But we can never induce REM sleep if the animal is awake. So the key question is, we need to find a method to induce the saliva sleep. In this study, we showed that we found that if we are not stimulate the garbage neuron in a tiny area, in a brain stem, we can immediately induce the saliva sleep. The opportunity is an invasive method. So it's not applicable to human. So scientists are developing the new method that may be non-invasive. For example, this shows that you can use the focus ultrasound that can open brain blood barrier that can permit the familiar drug to the specific site. In this way, they can control the drug delivery to the specific brain region so that they can specifically control animal behavior, maybe in the human. Thank you very much.