 The study investigates the antibacterial activity of fungal metabolite gliotoxin, GT, and its mechanism of action against gram-positive and negative bacterial species. It is found that GT-mediated growth inhibition is reversed by ZN2 plus or Cu2 plus addition, suggesting that zinc collation may be involved in the antibacterial activity of GT. The study also reveals that GT affects the bacterial proton, leading to intracellular re-profiling and dysregulation of Cu2 plus homeostasis. This article was authored by Shane G. Downs, Rebecca A. Owens, Ke'an Walshie, and others.