 we developed a computational approach to design uniform, high-intensity excitation light for low cost, quantitative fluorescence imaging of in vitro, x-vivo, and in vivo samples with a single device. Our methodology enabled us to optimize the illumination geometry for the purposes of imaging three differed fluorophores through a mammary window chamber model. Additionally, this approach was found to be more reliable than traditional methods and to enhance the local contrast of biological features. This article was authored by Rujia Wong, Reilly J. Deutch, Anakshi D. Sunasee, and others.