 HIV maturation inhibitors such as Biviromat, BVM, interfering with GAG processing are emerging as alternative antiretroviral drug candidates. Here, the authors report structures of assemblies of HIV-1 GAG fragments spanning the CAC terminal domain and SP-1 region bound to BVM. This article was authored by Sakari Tassaka, Knal Kay Zadresni, Roman Zadorosnyi, and others.