 Trichomonas vaginalis, the etiologic agent of the most common non-viral sexually transmitted infection worldwide, has an estimated annual prevalence of 276 million new cases. Mixed infections with different parasite strains are expected, although it is unknown how these strains interact with one another. It is known that parasites can communicate with their hosts to enhance their own survival and transmission, but evidence of mixed infections calls into question the extent to which unicellular parasites can communicate with each other. In this study, we demonstrated that different T-vaginalis strains can communicate through the formation of cytanine-like membranous cell connections. We showed that cytanines formed by an adherent parasite strain, CDC 1132, is affected in the presence of a different strain, G3 or B7RC2. Our findings suggest that this effect is contact independent and that extracellular vesicles, EVs, are responsible for at least part of the communication among strains. We found that EVs isolated from G3, B7RC2 and This article was authored by Nehu and Salas, Manuela Blosco-Pedreros, Tuandos Santos Mello and others. We are article.tv, links in the description below.