 African lions in a zoo in Indiana, USA were found to have been infected with SARS-CoV-2, leading to human exposure and potential transmission between humans and animals. The virus was detected in three people who came into close contact with the infected lions, indicating that the virus could be transmitted from animals to humans. This highlights the need for improved biosecurity measures at zoos, including regular testing of animals for the virus and development of rapid diagnostic tests for big cats. This article was authored by Audrey A. Segrist, Kira L. Richardson, Rhea R. Guy, and others.