 This research paper examined the differences between two methods for growing bacteria from plant material, a culture-dependent approach, CDA, and a culture-independent approach, CIA. The CDA focused on rice roots on four commonly used bacterial media, 10% and 50% TSA, plant-based medium with rice flour, nitrogen-free medium NGN and NFB, while the CIA assessed DNA extracted directly from root and rhizosphere samples. The authors found that the CDA mainly missed anaerobic, methylotrophic, methanotrophic and photosynthetic bacteria compared to the CIA, which could enable the design of ad hoc culture media and conditions to increase the rice-associated microbiota cult of ability. This article was authored by Musa Sando, Isawani, Agnieszka Klinowska, and others.