 Abstract hemoglobin E, Hb, Thalassemia is caused by a single-point mutation in the HbB gene, resulting in a severe form of the disease. This mutation can be corrected with a base editing technique, which re-established the asymptomatic trait phenotype. The technique has been successfully applied to both primary human CD34 plus cells and LTHSCS, and no off-target effects were observed. This article was offered by Mosin Badat, Ayesha Ijes, Penghua, and others.