 In this study, researchers used low-intensity pulsed ultrasound, lipos, to reduce the production of pro-inflammatory cytokines by M1 like macrophages. They tested various frequencies, intensities, duty cycles, and exposure times before determining the optimal conditions for decreasing cytokine levels. These conditions included 38 kHz frequency, 250 mW per square centimeter intensity, 20% duty cycle, and 90 minutes of exposure time. This resulted in increased metabolism and reduced ROS production without affecting cell viability. Additionally, they found that PAs01 and TRPV1 ion channels are involved in the modulation of cytokine release. Furthermore, they observed an enhancement of NF-Kappa-B signaling and an increase in actin polymerization. Transcriptomic analysis revealed that the effects of lipis on cytokine production are mediated by the P38MAPK signaling pathway. This article was authored by Francesco Iacoponi, Andrea Caffarelli, Francesco Fontana, and others. We are article.tv, links in the description below.