 This study optimized enzymatic hydrolysis conditions using response surface methodology, RSM, to improve the crystallinity of cellulosic fiber, resulting in a maximum increase of up to 14% in the Fiber Crystallinity Index, CRI. The treated fiber was further analyzed by field emission scanning electron microscopy, FESEM, thermogravimetric analysis, TGA, and compared with untreated fiber. Medium-density fiberboards, MDF, were manufactured from the optimum treated fiber, exhibiting improved tensile properties and lower water absorption properties as compared to MDF made from untreated fibers. This article was authored by Mohammed Nazir, Rokia Hashim, Offman Suleiman, and others.