 A novel platform for generating tetracycline, TET, inducible vectors that express artificial microRNAs in a dosage-dependent manner has been developed using synthetic biology principles. The vector generates a Paul II promoter-mediated artificial microRNA which was flanked by ribosome sequences, and the utility of this platform was proven by effectively silencing target genes and inducing anti-cancer effects in bladder cancer cell lines 5637 and T24. The TET inducible synthetic artificial microRNAs may represent a new therapeutic strategy for treating human bladder cancer. This article was authored by Yonghao John, Yu Chen Liu, Du Anhao Lin, and others.