 1. Plots are commonly used to compare sequence sets quickly and provide a synthetic overview of similarity. 2. Dgenies is a standalone and web application that performs large genome alignments using Minimap 2 software and generates interactive dot plots. 3. Dgenies allows users to sort query sequences, zoom in on the plot, download various files, and is an easy-to-install open-source software package developed in Python and JavaScript with source code available at and testing site at. This article was authored by Florio Cabanets and Christoph Klopp.