 Isolation and handling of microbial flora, for example, Lactobacillus and Acetobacillus, etc. First, introduction. Lactobacillus is basically gram-positive anaerobic. They are rod-shaped and non-spoir-forming bacteria. Lactococid bacteria have ability to form and carbohydrate into lactic acid. And milk is the rich source of Lactobacillus' dishes. That is why it is highly used for the identification of industrial probiotics. This practical is basically aimed for the isolation of Lactobacillus' stains present in the milk sample. Regents required MRS-EGGER, which is favorable for the growth of Lactobacillus' species. Micropipers, Tips, Burner, Sterile Petri Plate, Milk Sample, Labeling Marker, and Serial Dilutions of Sample. First, 5 ml of milk sample. Set the micropipers at 1 ml, which is equal to 2000 microlitre. Fill up milk sample into first test tube. Fix the sample by using similar procedure. Make 6 Serial Dilutions. After the preparation of Serial Dilutions, MRS-EGGER on Sterile Petri Plate pour the melted EGGER into the empty plate. EGGER should be evenly distributed throughout the plate. Now place the plate to dry. EGGER is solidified. We use 10 raised to minus 6 dilutions for inoculation of milk sample. EGGER is solidified. Now take 1 ml from 10 raised to minus 6 dilutions and pour on the surface of EGGER. Now use disposable glass spreader. Now carefully spread the inoculum on the surface of media. Keep spreading until inoculum is completely absorbed on the surface of EGGER. After spreading, place the plate in an incubator at 37 for overnight. 24 to 48 hours, growth of Lactobacillus fishy is appeared on the surface of EGGER plate. As shown, there are single isolated colonies of Lactobacillus, which will easily count with the help of colony counter. Identification needs biochemical test and gram straining, which we will proceed in the next video.