 This study used solexa sequencing to discover and analyze the expression patterns of micro-ionase, manas, in two soybean genotypes undergoing either water deficit or rust stress. The results indicated that there were 24 families of novel manas, six families of conserved manas, and 22 families previously reported in soybean. Additionally, the authors validated their findings by performing quantitative reverse transcriptase polymerase chain reaction, RTQPCR. This study provides valuable insight into the role of manas in water deficit and rust stress responses in soybean. This article was authored by Nacimento Leandroci, Abdel Norocadovi, Moselino-Gimores, Francis Marci, and others.