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Published on Jun 27, 2012
For more info, visit http://www.bio-rad.com/yt/1/V3-Wester.... This video illustrates the five-step process of Bio-Rad's V3 Western Workflow system. By combining traditional blotting techniques with innovative tools such as TGX Stain-Free™ precast gels, the Trans-Blot® Turbo™ system, and the ChemiDoc™ MP imager, this system allows you to quickly check electrophoresis results and blot transfer quality prior to western blotting, simplifying total protein quantitation analysis post-immunodetection. Obtain consistent results faster and more easily than ever before.
The Five Steps of the V3 Western Workflow system:
• Separate proteins — Mini-PROTEAN® and Criterion™ TGX Stain-Free precast gels offer superior protein separation in as little as 15 minutes in standard Tris/glycine running buffer • Visualize proteins — protein separation can be visualized and confirmed on a ChemiDoc MP imager after a 1-minute activation without staining. Stain-free technology is a sensitive, time-saving alternative to traditional Coomassie staining, offering a streamlined workflow • Transfer proteins — the Trans-Blot Turbo system enables rapid protein transfer in as little as 3 minutes while maintaining high transfer efficiency over a wide range of molecular weights • Verify protein transfer — the ChemiDoc MP imager paired with stain-free technology enables instant verification of protein transfer to the membrane • Validate and quantitate — the ChemiDoc MP imager and Image Lab™ software can be used to validate western blotting data by total protein normalization to housekeeping proteins, eliminating the need for stripping and reprobing. Image Lab software yields quantitative results instead of relative normalization