 I would have known everyone. I feel really honored to stand here. And the subject of my presentation is the recombinant Dengue virus to NS5, an effective antigen for diagnosis of Dengue infections. Dengue virus belongs to flu-aware dial families and are classified into four immunologically distinct serotypes, can cause disease ranging from non-specific fever illness to classic Dengue fever, Dengue hemorrhagic fever and Dengue shock syndrome among the DHF and DSS are often fatal. And Dengue fever is endermic in tropic and sabotropic climates. There are nearly 390 million Dengue virus infections that occur annually and over the past decades, Dengue fever has separated from less than nine to currently about 128 in epidemic countries. Dengue virus is transmitted by female mosquitoes, mainly Egypti and ancient Alps pictures. All the serotypes can be transmitted from human to human. And the individuals infected by any one serotype will have life-long immunity against re-infection of the same serotype, but only partially protected from other serotypes. Infections with a different serotype are associated with a higher risk of severe complications such as DHF and DSS due to ADE infection. And the Dengue virus is also a prototype of other flu viruses. For example, the Zika virus, this homology raises the risk of cross-reactive immune responses and subsequent ADE infections in the case of secondary infections or immunization of the individuals. And the recent outbreak of Zika virus epidemics has raises a growing concern with both viruses. Around early 2016, the first Dengue vaccine has been marketed in several countries, but accumulating response indicates that this vaccine did not achieve the desired effect. And it should take conscious that this vaccine, this vaccine might cause ADE infection in populations. Here are some prevention and control strategies are proposed and practiced in Dengue endemic regions, but none of them achieve the effect. Dengue diagnosis is based on epidemic information, clinical symptoms and combined laboratory detections although there is no specific treatment. Early diagnosis is important for proper medical care to minimize mortality and for proper initiation of public healthy control measures. There are several approaches to establish laboratory diagnosis. And these are all different specificity and sensitivity. And require more than two detections, two detection indicators. Any single indicators is not enough to provide reliable and effective results. And there are some rapid diagnosis of antigen detection and antibody detection. Targeting NS1 are now commercially available, but these both of them require all the four stereotypes of recombined NS1. Why are we interested in NS file? Dengue virus NS file are highly conserved among stereotypes that have not been found to participate in the ADE phenomenon. Dengue single open reading framework includes a polyprotein precursor which is cleared by cellular and viral properties into structural and non-structural properties. NS1 and NS file appear almost at the same time. And NS file consists of two major functional domains. The N-terminal ion method transfer is domain and the C-terminal ion dependent ion polymericis domain. And the NS file IDRP domain plays a vital role in viral ion replications like other polymericis. Dengue virus I process an architecture that is conserved across different classes and families of polymericis. This structure mimics a half closed right hand with the three sub-domain termed the finger palm and thumb. NS file potential to be an anti-viral target and a biomarker of Dengue diagnosis. We constructed an IDRP domain expression placemates and NS file four length expression placemates. And expressed a purified 70 keto ketone fragment and the NS file a four length fragment in E. coli. And these two purified recombinant products reacted with the serial operations effected with Dengue virus in an ELISA with higher OD index with compared with control zero. And the recombinant Dengue virus two NS file showed strong reactivity for any of the four serotypes while the 70 keto fragment show strong reactive for Dengue virus two and four. And the Dengue virus two NS file might be an infective antigen for Dengue diagnosis and the 70 keto fragment also can be used for auxillian diagnosis. Thank you.