 The results of this study suggest that the implementation of lockdown measures has had a negative impact on the well-being of female professional soccer players in Chile. Specifically, the players reported higher levels of muscle soreness, stress, fatigue, and mood during the lockdown compared to the regular preparation phase. Additionally, the players' perception of training load did not change significantly between the two phases. This suggests that further research is needed to assess the long-term effects of these measures on the physical and mental health of professional soccer players.