 Yeah today We're very close to harvest here, but there is one thing we've got to do because We want to be able to figure out what went wrong and what went well so we're going to count way Calculate the components of yield now the components of yield are and Lenny here is going to be my perfect assistance because she's doing this for like 10,000 samples each growing season No person who can do this better than that Components of yield are okay. You just basically the product of the number of Panicles per area. So let's say how many of these particles we've got on a square meter, okay? Multiplied by the number of Filled grains in a particle. Is that right? Yes, and then multiplied by the weight of a grain Now the weight of an individual grain is kind of hard to measure. So we usually count a thousand or you know Calculate a thousand grain weight. So if you multiply these three things and you adjust it to a standard moisture content of 14% that's usually how we express yield of a crop and by Quantifying by measuring these different components of years and there's many other details to that We can figure out then Probably some reasons for why we have gotten a high yield or low yield and also how variable the whole thing was all over this field So I was gonna do this in a very simple way So since we have a machine planted crop So I thought okay, I'm just gonna take it like a stick and like a meter You know and throw it somewhere randomly in the field wherever it lands I would put it on the row cut the rows and carry them out But not a good idea. No, that's not what we do. Why we have to be more scientific. So What I suggest is like I already prepared I know the size of this flat It's 12 meters by 100 meters So from that size we try to use the excel function to generate numbers random numbers random numbers So, why do you do that? I mean, I know I can kind of take a spot here and there You wouldn't trust me to pick the right spot. We want to avoid bias or like We need to get locations that are representative of the whole area and Using eight different locations by this generated numbers. I think that would capture the variability and To get the Yeah, okay, that's what we're gonna do. So use random locations and then we'll show you in a minute what we do at each place Okay, here is one of our random number generated locations. It says 54 meters from the end of the field five rows in all right. Yes, go find it. Where is it? So we need to count now One two three four five Okay, what we're gonna do here. I'm here here. So we have to have our Measuring device now Measuring device. Yeah, where is the measuring device guys? Yeah, okay. Good So we've got a tape here, so they are putting the tape where here we have to start from here Yeah, right between two plants right so that we don't bias the sampling And then you've got to make sure that the tape is nice and straight on the surface Yes, and then we count the number of plants from here one two three four five six seven eight nine ten And then we read the distance. What is the length? One million ninety One meter ninety again. No way. I think you got eleven points one two three four five six seven eight nine ten This one is the card. Oh, very nice. Yes one point nine. One million ninety in length ten plants Okay, we need to write down now in our data shift. Yeah one point nine. Let's cut them So now what we are doing is to the touch the panicle individually. Okay, we're gonna carry those out and then we'll process them Okay, good Got them ten, okay Yeah, but what I've noticed that I don't see too many White heads so stem borer damage seems to be low this time. I don't see too many disease problems. Would you agree? Yes, I haven't seen anyone actually good. That's reason for hope. So what do we see? We see different number of dealers like ranging from How many ten or less less than ten in some cases that's bad more than 20 and more than 20 like in this one But not all of them will have a panic. All right So are we gonna count all that stuff? We're going to count first we're going to count the number of dealers and then the number of panic L that way we will be able to know the field the tealering efficiency Tealering efficiency that will be the ratio of the panic L and the total number of dealers. What else are we gonna measure on this? We will here we will detach the panic L So we will partition the organs we will get the panic L dry weight blade dry weight and the stem dry weight Right, then we will get the total biomass and then later on we will separate the field and unfilled grains So the ratio of the field grains to the total biomass will be our harvest index That's a lot of work, but very very useful data that will make use of let's get going can't wait for these data They're coming We just need to put them in the oven for 72 hours at 70 degrees centigrade all of this. We will have a partition biomass and Then later you will soon have the date good. So this is the hybrid today the inbred we have to do the same kind of sampling in about five six days because it's behind in development and then we'll come back also in five or six days to Do the final harvest of that hybrid already? Yeah, so we will come back for the inbred We now bring our samples here in the lab to do detailed measurements We have to look for plant rates that would relate to yield. So that's why we are doing all these measurements First we have to be very careful not to lose any dead material So first we have to remove all the dead material and then afterwards We need to go to the washing room Remove the soil and then bring back again the samples in the lab and carefully remove the roots and We also have to have the data sheet where we will be putting the date because we need to count the tillers The tiller number We have to put it in our data sheet and then later on we also need to count the panicle number We need to determine the tillering efficiency at harvest So that's why we are counting the tillers This includes all productive and non-productive tillers and then afterwards We will do now our partitioning of the organs first is we need to remove the green blades and then afterwards remove the dead Dead blades and then the stem and we put them in their corresponding bags So after separation We need to put these samples in the oven at 72 degrees centigrade and We put these samples for three days and afterwards we will be Measuring and collecting the dry weights after counting the tillers. We also need to count the panicle number Since we sampled 10 plants, so we are counting the panicle number of the 10 plants We will then just get the average panicle number per plant later on so after separating the panicles the green leaves the stem We are now ready to put them in the oven set at 70 degrees centigrade and we will wait for three days before we take them out and Collect the dry weight using analytical balance from the oven We think we bring out the samples and wait until the temperature Equilibrates to room temperature and then we will then put the samples on this one and collect the dry weight