Table of Contents:
00:00 Lecture 4.3: Selectivity: When all else fails, tag, filer and amplify
01:50 Outline
02:20 Classical approach to selectivity
04:05 Increase the signal
05:16 Selectivity: A problem of Information theory?
07:39 Outline
07:44 Centrifuge improves SNR
09:02 Centrifuge isolates components by mass: Svedberg equation
12:13 The final step of differentiation
12:43 Outline
12:54 Idealized Polymerase Chain Reaction (PCR)
14:22 How PCR Doubles DNA Count
16:08 Untitled: Slide 13
16:45 Modeling of PCR reaction:
18:58 Untitled: Slide 15
23:01 Outline
23:42 Selectivity: A problem of Information theory?
23:52 Reduce noise: Optical tag-based sensing
25:21 Magnetic tag-based sensing
27:13 Conclusions
This video is part of the nanoHUB-U course "Principles of Electronic Nanobiosensors". (https://nanohub.org/courses/PEN)
This course provides an in-depth analysis of the origin of the extra-ordinary sensitivity, fundamental limits, and operating principles of modern nanobiosensors. The primary focus is the physics of biomolecule detection in terms of three elementary concepts: response time, sensitivity, and selectivity. And, it potentiometric, amperometric, and cantilever-based mass sensors to illustrate the application of these concepts to specific sensor technologies.
For more details see http://nanohub.org/u
