 The Golden Age of Antibiotics for Tuberculosis, TB, was marked by its success in the 1950s of the last century. However, TB is not under control and the rise in antibiotic resistance worldwide is a major threat to global health care. Understanding the complex interactions between TB Basili and their host can inform the rational design of better TB therapeutics, including vaccines, new antibiotics, and host-directed therapies. Recently, we demonstrated that the modulation of cystotin C in human macrophages via RNA silencing improved the anti-mycobacterial immune responses to mycobacterium tuberculosis infection. Available in vitro-transfection methods are not suitable for the clinical translation of host cell RNA silencing. To overcome this limitation, we developed different RNA delivery systems, DSs, that target human macrophages. Human peripheral blood-derived macrophages and THP1 cells are difficult to transfect using available methods. In this work, a new potential nanomedicine based on kytosan CSDSO was. This article was authored by David Perez, Manaj Mandal, Ana Aymados, and others. We are article.tv, links in the description below.