 The optimal enzymatic cocktail for hydrolyzing AFEX-pre-treated corn stover consists of a combination of two xylonases, LX3 and LX4, and three cellulases, Cbhi, Cbh2 and Egi, at a mass ratio of 25 to 75. Additionally, the addition of 0.6mg per G-glucon of xylosidase, LX, and 0.6mg per G-glucon of arabinofuranosidase. L-ARB increases the monomeric xylose yield by 20%, while 0.8mg per G-glucon of glucuronidase, LGL, further increases the xylose yield by another 20%. Furthermore, this cocktail has been shown to reduce the total enzyme loading required for equivalent yields when compared to Accelarase 1000, a commercially available enzyme cocktail. This article was authored by Hermannson Spencer, Usherung, Chanda Wachishi P.S., and others.