 Hi everyone, welcome to basic science series. This is the fourth lecture of our biochemistry lecture series, topic protein synthesis. In the previous lecture, we learned about H. Govind Kurana's experiments, what are open reading frames and what are start and stop codones. If you have any questions regarding the lectures, please type your questions in the comment section and I'll get back to you as soon as possible. Alright, without delay, let's start today's lecture. The topic of today's lecture is wobble hypothesis. The scientific understanding of codon and anti-codone base pairing provided an interesting hypothesis known as wobble hypothesis. It was given by Crick and it suggested four different relationships between codon and anti-codone base pairing. The first important point of wobble hypothesis is that the first two bases of mRNA molecule will be responsible for the strong Watson and Crick pairing. For example, in case of ADG, A and T will be responsible for the strongest binding. Taking an example of amino acid alanine where you have four different codones that codes for alanine and you can see the first two bases are same in all different codones. That suggests that first two bases are responsible for strong binding. Next important point is where you have one mRNA molecule. The codon of the mRNA molecule is in line with the anti-codone of the TRNA molecule and the first base of anti-codone and the third base of codon are responsible for the number of codon recognized by TRNA molecule. For example, in case of U or G, there will be some non-specific binding. In one codon scenario, if your anti-codon has C, your codon will have G and if your anti-codon have A, your codon will have U-resil. So you don't have any other options for this combination. In case of two codon scenario, your anti-codon, if have U that is U-resil, the codon can have an option of having an anine or guanine at the third position. If the anti-codon has G, then there is option of C and U. In the three codon scenario, if you have Inocene present in the TRNA anti-codon, then mRNA can have adenine, uresil or cytosine at the third base position. The third important point is when one amino acid has several codon, the difference in either first or two bases will require a separate TRNA molecule. The fourth important point is there are minimum 32 TRNA molecules for 61 codons and three codons as we know are stop codons. All right, this was all about the major points of the wobble hypothesis. If you have any questions, please ask those questions in the comment section below. I will try my best to answer those questions. Please do not forget to subscribe the YouTube channel. Thank you and namaste.