 The study examined the differences between the heat activation properties of TRPV1 proteins found in tailed amphibians and those found in rats. The researchers discovered that the heat activation threshold for the tailed amphibian TRPV1 was much lower than that of the rat protein. They also identified two amino acid residues in the anchor in repeat 1, Ank1, region of the N-terminal domain that were responsible for this difference. These results suggest that the cooled habitat preference of tailed amphibians may be due to their ability to sense lower temperatures more efficiently.