 HIT-SCISOSEC is a new technology that allows researchers to obtain high-quality data on single-cell INA isoforms at high throughput. It uses PAC-BIO CCS to combine multiple CDNAs from each cell, resulting in more than 10 million long reads per cell. This enables researchers to accurately identify and quantify the expression levels of different INA isoforms in each cell. The technology has been applied to study the transcriptome of corneal lumbus cells, revealing cell type, specific isoform expression. This article was authored by Xu Xingshe, Xi Chaoqian, Zhou Yongxiang, and others.