 antibiotic resistance and, accordingly, their pollution because of uncontrolled usage has emerged as a serious problem in recent years. Hence, there is an increased demand to develop robust, easy, and sensitive methods for rapid evaluation of antibiotics and their residues. Among different analytical methods, the aptimer-based biosensors, apti-sensors, have attracted considerable attention because of good selectivity, specificity, and sensitivity. This review gives an overview about recently developed apti-sensors for antibiotic detection. The use of various aptimer assays to determine different groups of antibiotics like N-beta, lactams, aminoglycosides, anthracyclines, chloramphenicol, fluoro, quinolones, lincosamide, tetracyclines, and sulfonamides are presented in this paper. This article was authored by Assal Melhorn, Parvana Rahimi, and Yvonne Joseph.