 Semuanya, saya Arfin. Dan owner ini. Jadi kita di sini, mahu berkongsi sedikit tentang tech saturday dan apa yang kita lakukan. Jadi projek kami di DIY bio. So tech saturday adalah meet up. Kita ada ini setiap saturday. Apa yang kita lakukan adalah kita mahu berkongsi kultur yang berlaku. Jadi segalanya yang kita lakukan, segalanya yang kita lakukan kita mahu berlainan ke sustainability. Mungkin kembali, sustainability, sosial, atau even money tree as well. We also collaboration. So what we do is a public program. So if you want to join us, it's just open for you. We meet every saturday at DRWC, UWC East. And what we want to do is to share our learnings. So today also is a means for us to share what we have learned, our mistakes and how we can then move on after this. So this is what we do usually. So we open up a project to public, can sign up, and we learn how to make stuff and then we make the things and you know, obviously it is not done. Later we'll talk about it. And then we share about what we have learned. And every week we have our updates in this Facebook group. So you can check out the maker circle. We have updates every week on our project. So today we are going to share a few two things. Number one is the incubator. The next one is the magnetic stirrer. For incubator, well it's a device that is used to grow and maintain microbiological cultures, cell cultures. So we have a space in our lab that is still empty. And we want to make it into a DIY biolab. And you know we have microscope and stuff like that but that's all we have. So we want to start everything from scratch. We want to build our own incubator. So yes, we need an incubator because there's a lot of biological things that requires temperature to be constant for it to be maximum. For it to for example enzyme reaction, DNA interaction and the stale state. For example, you want to grow bacteria. You want it to be about 37 degree Celsius, 40 degree Celsius so that it can grow the best. So to build our incubator, we first check out online. And we realize that there's this online course on on DIY bio by the W.A.G. or what, societies. It's BioHack Academy. So they run this from Netherlands. But they have centers in some part of the world like China. I think China have Tokyo, Korea and they run it. And we thought hey, this is good. We can try to get some of their designs and make it ourselves as well. So for incubator this is what they have. We we get inspiration from here and we just modify it according to what we have. For example, for the okay, for the cabinet itself some size consideration is the size of the petri dish. So you want a box that's big enough to put in about 9 cm diameter petri dishes. And at the same time, so you don't need to big because for ourself our lap is quite small. It's about 3 by 3 3 by 3 room space. So we don't want the incubator to be too big as well. And then for material we just get whatever we have in our lap. So 3 quarter inch wood sheet quite thick because we want the insulation. Ya the acrylic sheet is used for the for the window at the front with the door and we also have aluminium sheet to make it look more shiny. So for the heat source we were thinking what to use. Right, we have a few heat source that we can find around lying around our lap, you know. We have the soldering iron that we can hack and take the the heating element. We have the head dryer and so we have the this is the heating element from coffee maker and we decided that okay let's just use that. You know. So coffee save the day once more. For temperature control we use the Arduino as the as the brain. Alright. And we use relay to turn on and off the heat the heating element. And that two button is just to to change the target temperature. So we can change it 40, 35. So we we put on everything we put it inside and it worked. Right. So as you if you see here this is the temperature inside 43 right now degrees Celsius but it's not hot enough for now what we want to try or what is the maximum we can try. So we want to do rapid prototyping what we did is just insulating it by using tape. So we use tape and insulate everything around and we manage to get into 60. So right now the challenge for us is to insulate it with things other than tape. Alright. So our plan after this is we we are going to this is the freezing schematics that we make want to share it in instructables when it's done. So if you are interested can check out our instructables soon. Right. The next thing we want to share our donor will share is the DIY magnetic stirrer. So Great. Dona. Just doesn't actually. Yeah, it's okay. Choice. Okay. So for the incubator if you want a demo of it we will show it after this talk because it might take a little bit of time right now. Yeah. So moving on to the magnetic stirrer. So basically this little device here is what we made to basically stir liquids ideally it would have a heat pad as well because this will help with chemical reaction and whatnot. So yeah. So it's a laboratory device it emplies a rotating magnetic field. So over here actually you can't see it yet but inside there are two magnets and over here this stir bar which is now in two which is one would be spinning around when the fan inside rotates. So this will cause the spin liquid ends up spinning and we've got a method to control the speed as well. So this little DIY experiment of ours turn out quite well. So just to share a little bit about the process we wanted to it's important to actually control the speed of your spin this will depend on your different experiments. So what we did is that we used a single turn potential meter we used it as a real set you know this are just it's one of the ways you use a potential meter I guess most of you would understand this. Yeah so we wanted to use we wanted to adjust current flow which will help with controlling the fan speed. And initially the fan that we wanted to use was a salvaged it was salvaged from a CPU we were very excited about using something salvaged very cool Unfortunately it had an IC in it which meant that you know we found out by actually experimenting we wanted to turn it around positive and negative only in a certain direction it works the other way it didn't work so then we realised that our circuit wasn't working because having the IC meant that we could not use a potential meter with it. So we went to Simlim Tower and got ourselves a more simplified DC no integrated circuits we also got ourselves a high voltage battery 12V, very cool on our switch and our circuit works after that. Really simple setup so once we got our circuit working we basically went about building this little box and you'd be surprised how difficult it is to build this box with precision so this is, you know how many makers does it take to make one box I only quite a few So this is some people from the team that were helping out and yeah so over here on the bottom left you can see we were actually in a car park because there was a top going on at the time in the lab so it was a bit noisy so we actually moved the jigsaw out to the car park and continued our work because work never stops so yeah so after that we built this a little box and over here you can see a very simple basically what's inside because I can't open it up right now everything connected really well and we added a little light as well so that when you turn it on, it's pretty obvious so now let's see yeah so we're I don't know if you can see this but basically right now the battery has died so this is great okay okay so we changed the battery can you see this yeah it's not working but we might have to change the battery again so you can see it yeah you can see it sometimes with the liquid in it basically it just spins it's a really easy setup and the beauty of this is that you'll be surprised how many things in the biolab you can just make yourself you know wax with it what Arvind was talking about it's got all the instructions you need and if you want to join us come back on Saturdays yeah we're there from what 9 to 9 to end to depending on the project 12 or 10 p.m so yeah if you have any questions anything