 This study investigated the role of IpsiCd4 in the regulation of carotenoid accumulation in sweet potato tuberous roots. It was found that IpsiCd4 plays a critical role in the regulation of carotenoid accumulation in sweet potato. Furthermore, it was discovered that IpsiCd4 is regulated by two stress-related transcription factors, IpWrky20 and IpsiBf2, suggesting that the carotenoid accumulation in sweet potato is fine-tuned in response to stress signals. This study provides new insights into carotenoid metabolism in sweet potato tuberous roots and suggests IpsiCd4 as a potential target gene in the development of new sweet potato varieties with high carotenoid production. This article was authored by Jie Zhong, Lehen He, Jingjing Dong, and others.