 The study investigates the presence of RNA in exosomes from human saliva, plasma and breast milk, as well as their ability to be taken up by macrophages. Exosomes were purified using ultrason-trifugetiphogen and filtration steps, detected by electron microscopy and flow cytometry, and analyzed for the presence of RNA and specific proteins. The results show that RNA was detected in exosomes from all three body fluids, with a portion of the detected RNA in plasma exosomes being characterized as mRNA. Additionally, saliva and breast milk exosomes were found to be taken up by human macrophages. These findings extend the characterization of exosomes in healthy humans and support the notion that exosomal RNA can be shuttled between cells. This article was authored by Gabriel Saint-Suzanne, Stostrand Margarita, Bosio's Apostolos, and others. We are article.tv, links in the description below.