 The ready technique allows researchers to create large-scale libraries of oligonucleotides with high purity and uniformity, as well as easily access individual oligonucleotides from the pool. This makes it possible to rapidly and cost-effectively create strain collections from oligonucleotide pools, allowing for more efficient analysis of gene expression and other biological processes. This article was authored by Justin D. Smith, Ulrich Schlecht, Wei Hongshu, and others.