 The study presents a new method for preparing Illumina sequencing libraries from extremely AT-rich genomes, such as those of Plasmodium falciparum and Mycobacterium tuberculosis. The proposed method uses a PCR additive, TMAC, to amplify the DNA, which improves coverage of AT-rich regions and reduces bias towards GC-neutral templates. The study compares the quality of sequence data generated using the optimized conditions with standard methods and shows that the new approach significantly reduces bias and retains the complexity of both extremes of base composition. This development will benefit sequencing clinical samples that often require amplification due to low mass of DNA-starting material. This article was offered by oiler Samuel O., Otto Thomas D., G. U. Young, and others. We are article.tv, links in the description below.