 This study developed a new electrochemical detection technique for rapidly detecting bacterial pathogens such as Bacillus Sirius and Escherichia coli O157H7. The technique combines cyclic voltammetry with immunomagnetic separation and uses a magnetic-slash-polyanoline core-slash-shell nanoparticle to extract target cells from the sample solution and magnetically position them on a screen printed carbon electrode, SPCE. The presence of target cells significantly inhibits current flow between the electrically active core-slash-shell nanoparticles and the SPCE, allowing for quick detection of the pathogen. This technique has the potential to be adapted for a wide variety of target organisms in sample matrices and could become a fully portable system for routine monitoring or emergency detection of bacterial pathogens. This article was authored by Evangeline C. Alacilja and Emma B. Setterington.