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Development of a fly embryo in real time

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Published on Jun 7, 2012

Development of a fly embryo in real time (Video)
In an advance that could transform our understanding of the complex cellular dynamics that determine the development of animals, researchers have developed a method to track individual cells in a developing fly embryo in real time.

Multiview light sheet microscopy
Two independent groups—Keller and colleagues and Hufnagel and colleagues—report simultaneous multiview light-sheet microscopy using two illumination and two detection arms for high-resolution cell tracking and quantification in vivo. Both groups apply their methods to imaging the developing fly embryo.

- Fruitfly development, cell by cell
Multidirectional imaging of embryos allows researchers to track development of fruitflies in real time.
Nature 03 June 2012 DOI: doi:10.1038/nature.2012.10769
http://www.nature.com/news/fruitfly-d...

References
(1) Quantitative high-speed imaging of entire developing embryos with simultaneous multiview light-sheet microscopy
Nature Methods 03 June 2012 DOI: doi:10.1038/nmeth.2062
http://www.nature.com/nmeth/journal/v...

Abstract
Live imaging of large biological specimens is fundamentally limited by the short optical penetration depth of light microscopes. To maximize physical coverage, we developed the SiMView technology framework for high-speed in vivo imaging, which records multiple views of the specimen simultaneously. SiMView consists of a light-sheet microscope with four synchronized optical arms, real-time electronics for long-term sCMOS-based image acquisition at 175 million voxels per second, and computational modules for high-throughput image registration, segmentation, tracking and real-time management of the terabytes of multiview data recorded per specimen. We developed one-photon and multiphoton SiMView implementations and recorded cellular dynamics in entire Drosophila melanogaster embryos with 30-s temporal resolution throughout development. We furthermore performed high-resolution long-term imaging of the developing nervous system and followed neuroblast cell lineages in vivo. SiMView data sets provide quantitative morphological information even for fast global processes and enable accurate automated cell tracking in the entire early embryo.

- Supplementary information Video (1-20)
http://www.nature.com/nmeth/journal/v...

(2) Multiview light-sheet microscope for rapid in toto imaging
Nature Methods 03 June 2012 DOI: doi:10.1038/nmeth.2064
http://www.nature.com/nmeth/journal/v...

Abtract
We present a multiview selective-plane illumination microscope (MuVi-SPIM), comprising two detection and illumination objective lenses, that allows rapid in toto fluorescence imaging of biological specimens with subcellular resolution. The fixed geometrical arrangement of the imaging branches enables multiview data fusion in real time. The high speed of MuVi-SPIM allows faithful tracking of nuclei and cell shape changes, which we demonstrate through in toto imaging of the embryonic development of Drosophila melanogaster.

- Supplementary information Video (1-12)
http://www.nature.com/nmeth/journal/v...

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