 Hello everyone, welcome to the introduction video for the paper on the Potomac Profiling of Bert Seyler's Lachniformis, which reveals a stress-response mechanism in the synthesize of extracellular polymeric flucolins. This work was accomplished by Schaman University in cooperation with State University of New York and Binghamton, and published in biotech law written by Engineering. Here are our group members, Dr. He, Dr. Ni, Dr. Wang, Dr. Zhong, Dr. Sun, Mr. Yu, Mr. Chen, and Ms. Yin. The stream by Seyler's Lachniformis we used was isolated by our group with the number of CGMCC-2S76. In a previous study, it was found to produce extracellular polysaccharides, what's short for EPS. But when the culture condition was changed, the extreme could produce polygamma glutamic acid, also called gamma-PGA. Both polymers show high-fluorolating activities and potential applications of industries. That's our interest. This paper more focused on its metabolic mechanisms. The method we use is sweat mass spectrum, which can analyze the proton quantitatively and quantitatively. That we can discover the stress response mechanism during the synthesis of polymers. The stream was captured in LB EPS PGA media individually. From the figures, we can tell that the stream has different growth curves and methodologies under different mediums, which suggests that it's properly involved in different metabolic mechanisms. Compared to the intracellular proteins under LB medium, the up-regulated and down-regulated proteins under EPS and PGA medium were identified respectively. Among of these identified proteins, most of them related to either the synthesis of amino acid or the degradation of amino acid. For the amino acid degradation, the amino acid degradation due to the down-regulated enzyme, which are marked by the green and pink arrows in this figure. However, the synthesis of amino acid relates to the up-regulated proteins, which are shown in this figure. So far, it is supposed that the extracellular polymeric synthesis is closely related to the amino acid metabolism. The hypothesis was verified by quantitative PCR of selective genes, which was quite consistent with the results from differential proteins. Also, the intracellular free amino acid was detected. Compared to the sample from LB medium, the bacteria with a starvation of amino acid within both EPS and GMPGA medium. Above all, we can conclude that in order to mitigate the starvation of amino acid, bacillus lackniformis was likely to synthesize either EPS or GMPGA polymers by regulating the intracellular metabolism. Thank you for your attention.