 Esteem Toolbox 2.0 is a software package designed to simplify and automate spatial analyses in ecology, evolution, and genetics. It integrates ArcGIS and MaxEnt software, reducing the time needed to develop common solutions. The software also provides tools for parameterizing species distribution models, maximizing their discriminative abilities while minimizing overfitting. Additionally, it has universal analogs for use with any modeling software, such as tools for biodiversity quantification, generation of least-cost paths and corridors, and assessment of the significance of spatial randomizations. Finally, it offers generalized tools for batch processing and converting GIS data types or formats, making it applicable to any ArcMap user. This article was authored by Jason L. Brown, Joseph R. Bennett and Conor M. French. We are article.tv, links in the description below.