 This study combined mass spectrometry imaging, MSI, stable isotope labeling, and a spatial variant of isotopalog spectral analysis to map distributions of metabolites, nutrients, and metabolic turnover fluxes across the brains of mice harboring GL261 glioma, a widely used model for glioblastoma. The results revealed significant changes in metabolism compared to normal brain tissue, including increase to NOVO fatty acid synthesis and elongation fluxes. These findings suggest that metabolic reprogramming may be involved in the development and progression of this type of cancer. This article was authored by Michaela Schweigerhaber, Ethan Stankliffe, Tana Lakshmi S. Anbu Kuma, and others.