 The novel colorimetric apti-sensor for OTA detection was developed using gold nanoparticles, ONPs, as carriers for positively charged tetromethylrodamine, TAMRA, tagged apt-AMRA probes. The ONPs were used to increase the chances of silver reduction and deposition, which was catalyzed by the positive and negative charges between the TAMRA and the negatively charged silver lactate. This charge-effect assisted silver enhancement was confirmed by testing different lengths of apt-AMRA probes with TAMRA. The resulting OTA apti-sensor had a working range of 1x102, 1x106pg-mL and a detection limit of 28.18pg-mL. Additionally, the apti-sensor was successfully applied to real samples, demonstrating its accuracy and reliability. This article was authored by Xiaoyan Yan, Rong Huan, Lu Lu Xiong, and others. We are article.tv, links in the description below.